IsoPrimer: a pipeline for designing isoform-aware primer pairs for comprehensive gene expression quantification.

ABSTRACT:

Motivation

Eukaryotic genes can perform different functions by generating multiple transcripts through the alternative splicing mechanism. The accurate quantification of gene expression in specific conditions is important for functional assessment and requires an accurate PCR primer pair design to target all expressed alternative transcripts, a complex and prone-to-error task if performed manually.

Results

To efficiently address this task, we developed a pipeline, called IsoPrimer, to design PCR primer pairs targeting the specific set of expressed splicing variants of the genes of interest, to be used in quantitative PCR, e.g. in RNA-seq validation experiments. IsoPrimer, according to the level of expression of the splicing variants derived from an RNA-seq dataset, can: (i)

SUBMITTER: Filomena E 

PROVIDER: S-EPMC12311343 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature