A Reflux Linked GATA Factor Fulcrum Dictates Lineage Commitment Through GPRC5B During the Esophageal Dysplastic Transition.

ABSTRACT:

Background & aims

GATA family transcription factors are somatically variable (SV) in esophageal adenocarcinomas (EAC) and inducible by simulated reflux. Our study examines the mechanisms whereby GATA family members (GATA4, GATA6, and the atypical TRPS-1) influence oncogenesis during the Barrett's esophagus (BE) metaplasia-dysplasia transition preceding EAC.

Methods

RNAseq analyses of esophageal cell lines and lesion-derived adult stem cells (ASCs) in conjunction shRNA- or CRISPR-facilitated gene silencing, together with reanalysis of The Cancer Genome Atlas data, spatial transcriptomics, and organ-on-a-chip studies were used.

Results

Although a gastroesophageal reflux disease history positively correlated with GATA4/6 somatically variable and a columnar-associated gen

SUBMITTER: Abuhussein O 

PROVIDER: S-EPMC12311540 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature