Untargeted lipidomics profiling for halal authentication of meatball products from mixed meat sources.

ABSTRACT: Food adulteration with non-halal components, particularly in thermally processed meat products like meatballs, presents significant challenges for conventional DNA and protein-based detection methods. This study applies untargeted lipidomics using ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) combined with multivariate analysis (principal component analysis [PCA] and partial least squares-discriminant analysis [PLS-DA]) to authenticate halal status in meatball products. Lipid profiles were obtained from beef, goat, pork, and their mixtures, and analyzed under both electrospray ionization in positive mode (ESI+) and electrospray ionization in negative mode (ESI-) modes. Glycerophospholipids emerged as dominant lipid species. PCA and PLS-DA revea

SUBMITTER: Harlina PW 

PROVIDER: S-EPMC12311581 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature