Modulation of the HRAS1 I‑motif DNA Promoter Region by Dietary Plant Flavonoid Kaempferol.

ABSTRACT: I-motif (IM) noncanonical DNA structures exist in cellular environments and are implicated in various diseases, including aging, cancer, and neurological disorders, making them attractive targets for drug development. IM DNA can act as molecular switches regulating transcription, especially within oncogenic promoter and telomere regions. This study investigates the interaction between kaempferol (KAE), a dietary flavonoid, with multiple IM DNA configurations alongside duplex DNA using extensive spectroscopic and thermodynamic approaches. UV-vis absorption studies show a 26.67% hyperchromic effect and a notable 15 nm red shift in the absorbance maxima of KAE upon HRAS1 IM binding. Fluorescence measurements indicate a 3.7-fold increase in emission intensity and a 17 nm red shift, coupled wit

SUBMITTER: Bag S 

PROVIDER: S-EPMC12311655 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature