Lab-in-Syringe Automated Miniaturized Bioconjugation of Magnetic Beads with Anti-SARS-CoV2 Antibodies.

ABSTRACT: We present the first automated synthesis of magnetic immunosorbents (MIS) using a lab-in-syringe (LIS) platform, facilitating antibody bioconjugation to magnetic beads via carbodiimide-mediated covalent binding. This approach is an efficient, reproducible alternative to traditional manual methods, minimizing pipetting steps, vortexing, and incubation with a reduced handling bias. Utilizing a 1 mL syringe pump with a 12-port multiposition valve and an internal magnetic stir bar enables precise mixing, bead dispersion, and magnetic capture for consistent bioconjugate synthesis. The LIS platform achieved a 99.6% bead recovery with 0.4 mg of MIS (1 μm in diameter), outperforming the 83% recovery of manual techniques, and maintained an 83% recovery at reduced scales of 0.2 mg, surpassing manual

SUBMITTER: Svobodova Z 

PROVIDER: S-EPMC12311662 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature