Orthogonal conjugation of anchoring-dependent membrane active peptides for tuning of liposome permeability.

ABSTRACT: Liposomes are widely utilized in drug delivery systems to enhance pharmacokinetics and reduce side effects. Bioresponsive membrane-active peptides (MAPs) can modulate the release of encapsulated drugs from liposomes, improving therapeutic efficacy. However, achieving efficient and specific conjugation of MAPs to liposomes remains challenging, complicating translational efforts. Thiol-maleimide Michael addition is an attractive strategy for lipid conjugation of cysteine-containing MAPs but is hindered by thiol oxidation. Strain-promoted azide-alkyne cycloaddition (SPAAC) presents an alternative conjugation approach, yet its effects on liposome and peptide functionality are not fully understood. Here, we demonstrate how these two conjugation strategies influence peptide-liposome interactions

SUBMITTER: Iversen A 

PROVIDER: S-EPMC12311776 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature