ASAP1 Promotes Epithelial to Mesenchymal Transition by Activating the TGFβ Pathway in Papillary Thyroid Cancer.

ABSTRACT:

Background

Papillary thyroid cancer (PTC) is the most common type of thyroid malignancy. While the prognosis of PTC is generally favorable, some cases exhibit aggressive behavior, leading to metastasis and recurrence. ASAP1 (ArfGAP with SH3, ankyrin repeats, and PH domain 1), an ADP-ribosylation factor GTPase-activating protein, has been implicated in tumor metastasis. However, its role in PTC remains unclear.

Methods

ASAP1 expression in PTC was evaluated using TCGA and GEO databases. Studies in PTC cell lines (MDA-T32 and MDA-T85) included lentiviral-mediated knockdown and overexpression of ASAP1 to assess effects on epithelial-mesenchymal transition (EMT) marker expression, cell proliferation, and invasive capacity. TGFβ pathway activity was examined by p-SMAD2 Western blo

SUBMITTER: Song S 

PROVIDER: S-EPMC12311982 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature