Unveiling the role of PSD95 phosphorylation after traumatic brain injury: insights from phosphoproteomic analysis.

ABSTRACT:

Background

Traumatic brain injury (TBI) is a global public health problem. Pathophysiology of TBI remains unclear. Thus, methods of TBI treatment are limited. Phosphorylation plays a vital role in many neurological diseases, including TBI. As an emerging technique, phosphoproteomic has been widely applied in many fields.

Methods

Rats were subjected to controlled cortical impact (CCI) or divided to sham group. Protein was extracted from cortex, digested and labeled by iTRAQ. After undergoing mass spectrometry (MS), differential expressed phosphorylated sites (DEPSs) were identified, and proteins of these DEPSs were also listed. PSD95 was chosen as a target protein for further research. ZL006 was used to treat TBI rats. Nissl staining was applied to assess lesion volume, apopt

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12312315 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature