Quantifying axonal features of human superficial white matter from three-dimensional multibeam serial electron microscopy data assisted by deep learning.

ABSTRACT: Short-range association fibers located in the superficial white matter play an important role in mediating higher-order cognitive function in humans. Detailed morphological characterization of short-range association fibers at the microscopic level promises to yield important insights into the axonal features driving cortico-cortical connectivity in the human brain yet has been difficult to achieve to date due to the challenges of imaging at nanometer-scale resolution over large tissue volumes. This work presents results from multi-beam scanning electron microscopy (EM) data acquired at 4 × 4 × 33 nm³ resolution in a volume of human superficial white matter measuring 200 × 200 × 112 μm³, leveraging automated analysis methods. Myelin and myelinated axons were automatic

SUBMITTER: Tian Q 

PROVIDER: S-EPMC12312701 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature