Methodological establishment and diagnostic value of a multiplex fluorescent PCR assay for the detection of three fastidious respiratory pathogens.

ABSTRACT:

Background

Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are common fastidious bacteria responsible for respiratory tract infections, particularly in children and immunocompromised individuals. Due to their demanding growth requirements, traditional culture methods often yield low sensitivity and delayed results, posing challenges for early and accurate diagnosis.

Objective

To establish a TaqMan probe-based multiplex fluorescent PCR method for the simultaneous rapid detection and identification of three important respiratory fastidious pathogens: Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis.

Methods

By designing and optimizing TaqMan probes and primers targeting Streptococcus pneumoniae, Haemophilus influenz

SUBMITTER: Shi J 

PROVIDER: S-EPMC12312904 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature