Crystal structure of the fungal mannosyltransferase Och1 reveals active site primed for N-glycan binding.

ABSTRACT: The outermost layer of a fungi's cell wall serves as the organism's point of first contact with its environment, or host. Heavily glycosylated glycoproteins anchor a complex meshwork of branching mannose chains, forming the outer cell wall layer in most yeast and mold species. Outer mannan chains are composed of large polymannose branching glycans attached to the universal eukaryotic N-glycan GlcNAc2Man8 core. Synthesized in the endoplasmic reticulum, the core N-glycan is transferred to the Golgi apparatus, where the first fungi-specific reaction takes place. In the cis-Golgi, Och1 (Outer chain elongation 1) plays a central role in initiating outer mannan cell wall synthesis by transferring a single α-1,6-mannose residue to the N-GlcNAc2Man8 core. Playing a vital role in fungal biology, fu

SUBMITTER: Kelly ETR 

PROVIDER: S-EPMC12312940 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature