A Cyanobacterial Screening Platform for Rubisco Mutant Variants.

ABSTRACT: Rubisco is the main entry point of inorganic carbon into the biosphere and a central player in the global carbon system. The relatively low specific activity and tendency to accept O₂ as a substrate have made Rubisco an attractive but challenging target for enzyme engineering. We have developed an enzyme engineering and screening platform for Rubisco using the model cyanobacterium Synechocystis sp. PCC 6803. Starting with the Form II Rubisco from Gallionella, we first show that the enzyme can replace the native Form I Rubisco in Synechocystis and that growth rates become sensitive to CO₂ and O₂ levels. We address the challenge of designing a zero-shot input library of the Gallionella Rubisco, without prior experimental knowledge,

SUBMITTER: Hoffmann UA 

PROVIDER: S-EPMC12313052 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature