Free fatty acid biosynthesis precursors are involved in pollen-stigma interactions in <i>Brassica</i>.

ABSTRACT: Self-incompatibility (SI) is a complex molecular mechanism in flowering plants that prevents self-fertilization and promotes outcrossing. We conducted metabolome analysis of ornamental kale (Brassica oleracea var. acephala) pistils following SI and compatible pollination (CP), revealing significant alterations in lipid metabolism, particularly the accumulation of free fatty acid (FFA) metabolites during CP. Treatment of stigmas with acetyl-CoA and malonyl-CoA, key precursors in fatty acid (FA) synthesis, broke down SI and enhanced CP. Conversely, inhibiting acetyl-CoA carboxylase (ACCase), the rate-limiting enzyme in de novo FA synthesis, significantly reduced compatible pollen attachment and tube growth, highlighting the critical role of FA metabolism in mediating pol

SUBMITTER: Qin H 

PROVIDER: S-EPMC12313337 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature