Functional analysis of a UDP-glucosyltransferase gene contributing to biosynthesis of the flavonol triglycoside in tea plants.

ABSTRACT: Flavonol glycosides have many prominent benefits to human health and significant contributions to the growth and development of tea plant as well as the color and taste of tea infusion. In this study, a gene isolated from tea plant was found to encode a 52.2-kDa protein located on the plasma membrane and in the cytoplasm with activity of flavonol glycosyltransferase (CsFGT). The prokaryotically expressed recombinant CsFGT (rCsFGT) exhibited its main glucosyl transfer activity towards rutin to produce quercetin 3-O-β-d-glucopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→6)-β-d-glucopyranoside (Q-g-r-g), and showed a minor galactosyl transfer activity towards delphinidin to produce delphinidin 3-O galactoside. The maximum activity of rCsFGT was observed at 30°C and pH 8.0. The main fun

SUBMITTER: Zhang WW 

PROVIDER: S-EPMC12313340 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature