Deciphering the role of SEMA4A/MAPK signaling in sepsis: insights from Mendelian randomization, transcriptomic, single-cell sequencing analyses, and vitro experiments.

ABSTRACT:

Background

Sepsis is a condition with high mortality and multiple organ dysfunction, undergoing complex pathogenesis and limited treatment options. This study aims to uncover new therapeutic targets for sepsis.

Methods

Three independent transcriptomic datasets from sepsis patients in the GEO database were utilized. Batch effect correction and differential gene expression analysis were performed to identify differentially expressed genes (DEGs), followed by mendelian randomization (MR) analysis to identify sepsis-related risk genes. The intersection of DEGs and MR risk genes revealed final core sepsis genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to elucidate the functional pathways of core genes. Single-cell R

SUBMITTER: Pei MQ 

PROVIDER: S-EPMC12313560 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature