Gut microbiota and protein-to-protein ratios in NAFLD: insights from Mendelian randomization and murine studies.

ABSTRACT:

Background

Gut microbiota and protein metabolism play critical roles in non-alcoholic fatty liver disease (NAFLD) progression, but their causal relationships remain unclear. This study integrates Mendelian randomization (MR) analysis and experimental validation to identify microbial and molecular contributors to NAFLD and explore potential therapeutic targets.

Methods

Two-sample MR analysis was performed to assess the causal effects of gut microbiota and protein-to-protein ratios on NAFLD using inverse variance-weighted, maximum likelihood, MR-Egger, weighted median, weighted mode, and Wald ratio methods. Sensitivity analyses were conducted to ensure result robustness. Mediation analysis was applied to examine whether protein-to-protein ratios mediate the link between gut mi

SUBMITTER: Lei L 

PROVIDER: S-EPMC12313671 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature