Impact of α-synuclein fibril structure on seeding activity in experimental models of Parkinson's disease.

ABSTRACT: The central pathogenesis of Parkinson's disease involves the misfolding and aggregation of α-synuclein (α-syn). There is a widespread belief that α-syn can propagate in a prion-like manner, and α-syn preformed fibrils (PFFs) have been widely used to establish α-syn propagation models. However, achieving standardized protocols for generating PFFs is challenging due to the influence of various factors on propagation efficiency, resulting in inter-laboratory and inter-experimental variability. Among these factors, the size of the PFFs is considered the most influential as unsonicated PFFs exhibit limited seeding and propagation abilities. Therefore, the objective of our research is to examine the impact of the size and conformation of sonicated PFFs on seeding activity. PFFs were sonicated un

SUBMITTER: Ohira J 

PROVIDER: S-EPMC12313852 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature