Deciphering antigen-specific T cell navigation tactics and cancer immune evasion in co-cultures.

ABSTRACT: Insufficient infiltration of cytotoxic T cells into solid tumors remains a critical obstacle in cancer immunotherapy. Despite extensive efforts to comprehend the mechanisms governing this limited infiltration, few studies have focused on the evolution of T cell motility behavior after co-culture. In this study, we combined quantitative cell trajectory analysis, computational modeling, and bulk/single-cell RNA sequencing to systematically characterize the impact of cell interactions. We reveal that in a 2.5D co-culture system with multiple cancer-cell clusters, cancer-specific T cells exhibit increased directional persistence, which facilitates their efficient searching of cancer-cell clusters. Additionally, these T cells form prolonged interactions with cancer cells, which is the most cruc

SUBMITTER: Li X 

PROVIDER: S-EPMC12313858 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature