Integrated transcriptomic and functional modeling reveals AKT and mTOR synergy in colorectal cancer.

ABSTRACT: Colorectal cancer (CRC) treatment remains challenging due to genetic heterogeneity and resistance mechanisms. To address this, we developed a drug discovery pipeline using patient-derived primary CRC cultures with diverse genomic profiles. These cultures closely resemble certain molecular characteristics of primary and metastatic CRC, highlighting their promise as a translational platform for therapeutic evaluation. Importantly, our engineered model and patient-derived cells reflect the complexity and heterogeneity of primary tumors, not observed with standard immortalized cell lines, offering a more clinically relevant system, although further validation is needed. High-throughput screening (HTS) of 4255 compounds identified 33 with selective efficacy against CRC cells, sparing normal, he

SUBMITTER: Duleba M 

PROVIDER: S-EPMC12313885 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature