A cytokine-based designer enzyme with an abiological multinuclear metal center exhibits intrinsic and extrinsic catalysis.

ABSTRACT: A designer enzyme consisting of an abiological molecule incorporated into a natural protein has been developed as an exceptionally chemoselective catalyst, highlighting that the internal space of proteins is highly beneficial for enhancing catalytic performance. However, other features of proteins have received less attention in designer enzymes, for e.g., their use as ligands to construct abiological (multinuclear) metal centers and their intrinsic functions that have often been traded off for a new function. Here, grafting a synthetic trinuclear zinc complex inside a human cytokine macrophage migration inhibitory factor (MIF) scaffold using solely amino-acid side chains leads to a designer multi-metalloenzyme with extrinsic and intrinsic functions. The crystal structure of the designer t

SUBMITTER: Ueno A 

PROVIDER: S-EPMC12313933 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature