Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation.

ABSTRACT: Cryopreservation by vitrification could transform fields ranging from organ transplantation to wildlife conservation, but critical physical challenges remain in scaling this approach from microscopic to macroscopic systems, including the threat of fracture due to accumulated thermal stresses. Here, we provide experimental and computational evidence that these stresses are strongly dependent on the glass transition temperature [Formula: see text] of the vitrification solution, a property which, given the narrow band of chemistries represented within common vitrification solutions, is seldom investigated in thermomechanical analyses. We develop a custom cryomacroscope platform to image glass cracking in four aqueous solution chemistries spanning > 50 °C in [Formula: see text]; we process the

SUBMITTER: Kavian S 

PROVIDER: S-EPMC12314009 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature