Identification of novel human IgE-binding peptides from a phage display library for total IgE detection.

ABSTRACT: Immunoglobulin E (IgE) plays a key role in allergic reactions and parasitic infections. Accurate detection of IgE is essential for the diagnosis and management of allergic diseases. Traditional detection methods, such as enzyme-linked immunosorbent assay (ELISA) and radioallergosorbent tests (RASTs), depend on complex antibody production processes. This study aimed to discover novel peptides that specifically bind to human IgE using phage display technology. A 12-mer phage-displayed peptide library was screened against native human IgE, resulting in the identification of sixteen high-specificity phage clones from an initial pool of 208 candidates. Six of these clones were selected for peptide synthesis and further evaluation using multiplex assays. All synthetic peptides demonstrated speci

SUBMITTER: Kaew-Amdee S 

PROVIDER: S-EPMC12314100 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature