Cell specific perfusion rates drive growth dynamics and metabolism in CHO N-1 perfusion processes independent of perfusion rate control method.

ABSTRACT: The production of monoclonal antibodies (mAbs) using Chinese Hamster Ovary (CHO) cell host systems often faces challenges in terms of manufacturing costs and efficiency. To address these challenges, process intensification with high seeding density production processes applying N-1 perfusion is utilized. This study delves into the impact of cell specific perfusion rate (CSPR) and the total exchanged medium in relation to the reactor working volume (iVVD) on cell growth dynamics and metabolic stability in N-1 perfusion cultivations. The effect of varying the perfusion rate increase (PRI) while keeping the amount of exchanged medium constant is investigated, revealing a positive correlation between iVVD and overall cell growth. However, this effect plateaus at higher values, indicating dimin

SUBMITTER: Walther J 

PROVIDER: S-EPMC12314661 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature