Single-Entity Resolution Single-Cell Nanosensor Reveals Reactive Oxygen Species at Stress Granules Are Formed by Interfacial Redox Chemistry.

ABSTRACT: The electrochemical activities of biomolecular condensates represent a new fundamental functioning mechanism in biochemistry and cell biology. However, our understanding of the underlying molecular mechanism and the interfacial field-dependent chemical activities remains limited. This is due to the lack of technology to probe such activities in real time and at a single-condensate level. Stress granules (SGs) are membraneless organelles that form in the cytoplasm to adapt to cell stress, which are found to encapsulate reactive oxygen species (ROS) in our lab. Here, we design and implement a collision-based electrochemical nanosensor that enables probing of the redox activities of SGs at a single-condensate level in live cells. We show that ex-vivo separated SGs drive the redox reactions de

SUBMITTER: Gu H 

PROVIDER: S-EPMC12314916 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature