Investigation of biomolecular dynamics by sensitivity-enhanced ¹H-²H CPMAS NMR using matrix-free dynamic nuclear polarization.

ABSTRACT: Molecular dynamics of functional groups contain valuable information about structural properties and functional activities in biomolecules. NMR spectroscopy is a sensitive tool for the investigation of molecular dynamics over a wide range of timescales and thus may deepen the understanding of the biomolecules of interest. Here, we present an approach to use DNP-enhanced ²H NMR to study dynamics of selectively deuterated methyl groups in insoluble proteins such as amyloid beta (Aβ) fibrils. We adopted and optimized the matrix-free DNP approach by varying the amount of added polarizing agent as well as the rehydration level of model proteins. We show that the DNP enhancement obtained in ¹H-²H cross-polarization (CP) MAS spectra may increase the sensitivity fo

SUBMITTER: Biedenbander T 

PROVIDER: S-EPMC12315109 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature