Adipocyte exosome miR-4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D.

ABSTRACT:

Aims/introduction

Previous studies have found that miR-4472 is overexpressed in the serum of individuals with obesity and type 2 diabetes mellitus (T2DM), which may participate in the process of obesity-induced T2DM. However, a role for miR-4472 in the process has not been demonstrated. Here, we aim to investigate whether the increased content of miR-4472 in adipose tissue derived from exosomes inhibits glucose uptake in skeletal muscle by downregulating the expression of its target gene.

Materials and methods

In vitro C2C12 and 3T3-L1 cells, and in vivo diet-induced obesity mouse models and AT-Dicer KO mice were used to assess the impact of miR-4472 on glucose uptake and insulin sensitivity. We also evaluated the effects of serum exosomes from normal and obese individuals o

SUBMITTER: Sun C 

PROVIDER: S-EPMC12315254 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature