Screening and identification of host factors interacting with the nucleocapsid protein of SARS-CoV-2 omicron variant using the yeast two-hybrid system.

ABSTRACT:

Background

The nucleocapsid protein (N protein) of SARS-CoV-2 is highly conserved in viral evolution and serves as the primary structural protein in viral infection, being the most abundant in viral particles. The N protein is highly immunogenic and plays a key role in the processes of viral infection and replication, making it of significant research value in both basic studies and clinical applications.

Results

To further investigate the functions of SARS-CoV-2 N protein, the Matchmaker Gold Yeast Two-Hybrid System was used to identify potential interacting partners of the N protein in human peripheral blood mononuclear cells (PBMCs). Through this approach, we identified 11 host proteins that might interact with the SARS-CoV-2 N protein. We further validated the interactio

SUBMITTER: Wang C 

PROVIDER: S-EPMC12315389 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature