Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids.

ABSTRACT:

Background

Neuronal spheroids represent an easy and versatile solution to model neuronal tissue in vitro. Conventional approaches to generate spheroids lack accurate size control, scalability, and customizability. This is even more exacerbated in case of pluripotent stem cell (PSC) derived spheroids, which remain challenging to standardize. Microwell devices address these limitations, providing an optimal balance between accessibility and scalability. With the aim of optimizing culture conditions, we parametrically investigated the role of microwell geometry on the formation and maturation of iPSC-derived motor neuron precursor (MNP) spheroids.

Methods

We developed a customizable mold device using Digital Light Processing (DLP) 3D printing to fabricate agarose microwell arra

SUBMITTER: Mello E 

PROVIDER: S-EPMC12315449 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature