Exploring the molecular mechanism of OsROS1a in regulating resistance to bacterial leaf streak through transcriptome and DNA methylation profiling in rice (Oryza sativa L.).

ABSTRACT:

Background

DNA demethylases regulate the levels of genomic DNA methylation in plants. The demethylase REPRESSOR OF SILENCING 1 (ROS1) is a crucial factor for modulating gene expression in plant disease responses. Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. Oryzicola (Xoc), is a highly destructive disease in rice. BLS resistance in rice is known to be quantitatively inherited, but the mechanisms by which DNA methylation controls BLS resistance remain poorly understood.

Results

In this study, we knocked down OsROS1a expression in the rice variety Nipponbare using RNA interference (RNAi). The average BLS lesion length in the transgenic (T2) OsROS1a-RNAi (RS) lines was significantly reduced compared to that in wild-type Nipponbare plants (NP). Using whole-genom

SUBMITTER: Xie X 

PROVIDER: S-EPMC12315458 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature