Proteome study of dikaryotic and monokaryotic mycelia reveals the transfer between biological processes contributing to dikaryon growth advantage of the medicinal fungus Ganoderma lucidum.

ABSTRACT:

Background

Ganoderma lucidum, a well-known medicinal fungus in traditional Chinese medicine, exhibits a superior growth rate in its dikaryotic mycelia compared to monokaryons, and this is essential for its fruiting body formation and medicinal utilization. However, the biological process landscape contributing to the dikaryon growth advantage remains largely unexplored. This study performed Tandem Mass Tag (TMT)-based proteomic analysis of the dikaryotic mycelia and two compatible monokaryotic mycelia to elucidate the mechanism underlying this growth advantage.

Results

Dikaryotic mycelia showed superior growth rates over monokaryons in both solid and liquid cultures. Proteomic analysis revealed a remodeled proteome of the dikaryon, with elevated expression levels of proteins

SUBMITTER: Yu H 

PROVIDER: S-EPMC12315474 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature