A high-resolution data set of fatty acid-binding protein structures. I. Dynamics of FABP4 and ligand binding.

ABSTRACT: Fatty acid-binding proteins (FABPs) are involved in the uptake and intracellular trafficking of fatty acids for metabolic and gene-regulatory purposes. FABPs are known to associate with membranes and also enter the nucleus. Using NMR and a human FABP4 (hFABP4) preparation completely free of endogenous ligands, we studied the influence of fatty acids and inhibitors on the conformational flexibility and bicelle/membrane association of this isoform. Binding of fatty acids and ligands rigidifies hFABP4, particularly at the portal region where ligands enter the binding site. Depending on the nature of the ligand, hFABP4 stays associated with bicelles via the portal region or segregates into solution, a prerequisite for nuclear import using a nonclassical nuclear localization signal. These resul

SUBMITTER: Casagrande F 

PROVIDER: S-EPMC12315583 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature