Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

ABSTRACT: Three-dimensional (3D) structure determination by single-particle analysis of cryo-electron microscopy (cryo-EM) images requires ab initio 3D reconstruction of density volume(s) from 2D images (particles). This large-scale inverse problem requires the determination of many million degrees of freedom from extremely noisy experimental measurements. Here, we introduce a new approach to probabilistic multi-volume ab initio 3D reconstruction for simultaneous estimation of the relative particle 3D orientations and partitioning of the particles into groups with distinct structural states. To account for further structural variability within the discrete state groups, due to for example regional disorder, flexibility or partial occupancy of associating ligands, we introduce a new method for adapti

SUBMITTER: Van CTS 

PROVIDER: S-EPMC12315586 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature