Dynamic barriers modulate cohesin positioning and genome folding at fixed occupancy.

ABSTRACT: In mammalian interphase cells, genomes are folded by cohesin loop extrusion limited by directional CTCF barriers. This process enriches cohesin at barriers, isolates neighboring topologically associating domains, and elevates contact frequency between convergent CTCF barriers across the genome. However, recent in vivo measurements present a puzzle: reported CTCF residence times on chromatin are in the range of a few minutes, whereas cohesin lifetimes are much longer. Can the observed features of genome folding result from relatively transient barriers? To address this question, we develop a dynamic barrier model, where CTCF sites switch between bound and unbound states. Using this model, we investigate how barrier dynamics would impact observables for a range of experimental genomic and im

SUBMITTER: Rahmaninejad H 

PROVIDER: S-EPMC12315716 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature