GD2-mediated impairment of macrophage phagocytosis drives pulmonary metastasis in osteosarcoma.

ABSTRACT: Rationale: The lung is the most common site of metastasis in osteosarcoma, with pulmonary dissemination accounting for most of the disease-related mortality. Despite its clinical significance, the underlying mechanisms is poorly defined. Methods: To investigate the clinical relevance of GD2, we performed GD2 immunofluorescence staining on a cohort of human tumor samples. To explore the functional role of GD2 in lung metastasis, we employed an intravenous injection model and an intratibial injection model using U2OS and 143B cells respectively. To elucidate how GD2 regulates osteosarcoma lung metastasis, we carried out an in vitro flow-based phagocytosis assay. Results: We identify the disialoganglioside GD2 as a key mediator of osteosarcoma lung metastasis throu

SUBMITTER: He Y 

PROVIDER: S-EPMC12315822 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature