Comparative Analysis of Age-Associated Changes in Meibum Composition, Distribution, and Function in Mice With Altered Hyaluronan Expression.

ABSTRACT:

Purpose

Mice lacking hyaluronan (HA) synthase 1 and 3 (Has1-/-; Has3-/- mice) are resistant to meibomian gland (MG) atrophy and dropout. Herein, we characterized the composition and distribution of meibum in Has1-/-; Has3-/- mice as they age and verified whether they are protected from developing dry eye disease (DED).

Methods

Tarsal plates from wild-type (wt) and Has1-/-; Has3-/- mice were isolated, and meibum lipid composition and the distribution of meibum were analyzed by liquid chromatography-mass spectrometry and whole-mount Sudan IV staining, respectively. The expression of biosynthetic enzymes for major lipid populations was analyzed by real-time PCR. The efficacy of the meibum at stabilizing the tear film was assessed using the benzalkonium chloride dry eye model, a

SUBMITTER: Verma S 

PROVIDER: S-EPMC12315927 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature