Bidirectional Phosphorylation Changes in Opsins Associated With Early Myopia and Hyperopia Signal Regulation by Phosphoproteomics.

ABSTRACT:

Purpose

The study aimed to investigate the role of post-translational modifications (PTMs), specifically phosphorylation, in the pathogenesis of lens-induced myopia (LIM) and lens-induced hyperopia (LIH).

Methods

This study used an untargeted phosphoproteomics approach to identify more than 12,000 phosphorylation sites in chick retinas. The changes in phosphorylation levels were quantified using the tandem mass tag (TMT) technique. Furthermore, targeted mass spectrometry was employed to characterize and validate the phosphorylation changes in visual opsins.

Results

The analysis identified differential phosphorylation at specific sites: S334 in rhodopsin, S328 in violet-sensitive opsin, and S342 in blue-sensitive opsin. Notably, these serine residues were dephosphoryla

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12315934 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature