Extracellular vesicle-mediated spinal cord-brain crosstalk induces hippocampal neurogenesis impairment and cognitive deficits post-spinal cord injury.

ABSTRACT: Rationale: Spinal cord injury (SCI) is well-documented for its devastating impact on motor and sensory functions. However, its potential effects on cognitive function remain underexplored. This study aims to investigate the mechanisms of SCI-induced cognitive dysfunction, focusing on spinal cord-hippocampal communication mediated by extracellular vesicles (EVs). Methods: Cognitive function and hippocampal neurogenesis were assessed in mice subjected to either SCI or sham surgery. EVs were isolated from spinal cord tissues of SCI and sham groups and stereotactically injected into the hippocampus to evaluate their effects on cognition and neurogenesis. Cx3cr1-CreERT2 transgenic mice combined with AAV-CD63-EGFP injection were used to confirm the source of EVs. High-throughput se

SUBMITTER: Qin T 

PROVIDER: S-EPMC12316029 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature