Shifting redox reaction equilibria on demand using an orthogonal redox cofactor.

ABSTRACT: Nature's two redox cofactors, nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺), are held at different reduction potentials, driving catabolism and anabolism in opposite directions. In biomanufacturing, there is a need to flexibly control redox reaction direction decoupled from catabolism and anabolism. We established nicotinamide mononucleotide (NMN⁺) as a noncanonical cofactor orthogonal to NAD(P)⁺. Here we present the development of Nox Ortho, a reduced NMN⁺ (NMNH)-specific oxidase, that completes the toolkit to modulate NMNH:NMN⁺ ratio together with an NMN⁺-specific glucose dehydrogenase (GDH Ortho). The design principle discovered from Nox Ortho engineering and m

SUBMITTER: Aspacio D 

PROVIDER: S-EPMC12316058 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature