Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation.

ABSTRACT: The preimplantation period of embryonic development can be a key window for programming of postnatal development because extensive epigenetic remodeling occurs during this time. It was hypothesized that modification of one-carbon metabolism of the bovine embryo by addition of the methyl-donor choline to culture medium would change postnatal phenotype through epigenetic modification. Embryos produced in vitro were cultured with 1.8 mM choline chloride or control medium. Blastocysts were transferred into females and pregnancy outcomes and postnatal phenotype of the resultant calves determined. Exposure of embryos to choline increased gestation length and calf birth weight. Calves derived from choline-treated embryos were also heavier at weaning and had increased ratio of body weight to hip h

SUBMITTER: Estrada-Cortes E 

PROVIDER: S-EPMC12316091 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature