Mechanism of Astragaloside-Brucea javanica oil nanoemulsion against oral squamous cell carcinoma through CDK1/MTFR2: Network pharmacology, bioinformatics, and experimental studies.

ABSTRACT:

Objective

This study aims to investigate the core target of Astragaloside-Brucea javanica oil nanoemulsion (AS/BJO-NEs) against oral squamous cell carcinoma (OSCC) through network pharmacology, bioinformatics, and in vivo/in vitro experiments, elucidating its effects on epithelial-mesenchymal transition (EMT) mediated via MTFR2 and the underlying molecular mechanisms.

Methods

By integrating network pharmacology and weighted gene co-expression network analysis, critical gene modules linked to tumor phenotypes, EMT-related adverse prognosis, and elevated MTFR2 expression were identified, pinpointing the core target gene. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Gene Set Enrichment Analysis, molecular docking and molecular dynamics simulation were utilized for an

SUBMITTER: Lai Y 

PROVIDER: S-EPMC12316279 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature