Anti-β2glycoprotein I-induced neutrophil extracellular traps cause endothelial activation.

ABSTRACT:

Objective

Neutrophil extracellular traps (NETs) involvement in antiphospholipid syndrome (APS) pathogenesis is known, but the role of anti-β2glycoprotein I (aβ2GPI) antibodies-induced NETs in triggering a procoagulant and proinflammatory phenotype in endothelial cells (ECs) remains to be evaluated. This study investigated whether NET-aβ2GPI can activate ECs and whether NET-aβ2GPI and NET-phorbol myristate acetate (PMA) have different proteomic profiles.

Methods

Healthy donor (HD) neutrophils were stimulated with APS-aβ2GPI, normal human IgG or PMA. NETs were stained with anti-neutrophil elastase and 4'6-diamidino-2-phenylindole (DAPI), and the ability of aβ2GPI to bind NETs and inhibit DNA degradation was investigated. Following aβ2GPI, NET-aβ2GPI and NET-PMA stimuli, we eva

SUBMITTER: Mancuso S 

PROVIDER: S-EPMC12316379 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature