Highly Sensitive DNA Testing of Fusobacterium nucleatum in Colorectal Tumors.

ABSTRACT:

Background

Fusobacterium nucleatum (Fn) has been associated with the risk of colorectal cancer, poor colorectal cancer survival, and tumor attributes. Accurate and sensitive detection of Fn in tumor tissue is critical for evaluating their role in colorectal cancer.

Methods

We developed a droplet digital PCR (ddPCR) assay for detecting Fn using the transcription termination/antitermination gene (nusG) normalized for host tissue (solute carrier organic anion transporter family member 2A1). We assayed Fn(nusG) in matched tumor and normal tissues for 613 participants in the Seattle site of the Colon Cancer Family Registry. We used logistic regression to determine the odds of Fn enrichment in tumor tissue according to the tumor site and stage, adjusting for age, sex, and body mas

SUBMITTER: Hullar MAJ 

PROVIDER: S-EPMC12316549 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature