Optimizing antibody PET imaging: a comparative preclinical analysis of nanobody and minibody-like PET tracers.

ABSTRACT:

Purpose

Positron emission tomography remains the most important medical imaging technique for noninvasive imaging. Advances in radiotracer design, particularly those based on antibody(-formats), have broadened diagnostic applications. While minibodies and nanobodies stand out among antibody-type radiotracers due to their unique characteristics and pharmacokinetics, a direct comparison between these formats remains unexplored. In this study, we generated a so-called minabody, a minibody-like protein consisting of a nanobody fused to a CH3 domain, and compared it with its nanobody counterpart in vitro and in vivo upon labeling with various PET radionuclides.

Methods

A previously developed human TIGIT-targeting nanobody was reformatted to a minabody format to allow head-to-head

SUBMITTER: Zeven K 

PROVIDER: S-EPMC12316738 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature