A multicellular self-organized probiotic platform for oral delivery enhances intestinal colonization.

ABSTRACT: Stable gut colonization of probiotics is essential for sustained therapeutic effects, however traditional oral probiotic supplements often fail to adapt to the gut environment. Here, based on the observation that multicellular microcolonies instead of planktonic bacteria display a more advantageous gene pattern for colonization, we design a system encapsulating multicellular self-organized probiotic microcolonies, termed Express Microcolony Service (EMS), for efficient oral delivery and enhanced gut colonization of probiotics. Utilizing the stress-relaxing and acid-resistant property of the covalent-ionic crosslinking alginate hydrogel microsphere, the micro-cargo provides tunable nutrient supply and extracellular matrix support to facilitate microcolony self-organization. Notably, we show

SUBMITTER: Liu H 

PROVIDER: S-EPMC12317005 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature