Ligand-Based CAR-T Cells Targeting EGFR Exhibit Favorable Antitumor Effects Against Gynecologic Malignancies.

ABSTRACT: Epidermal growth factor receptor (EGFR) has been reported to be overexpressed in gynecologic malignancies. However, the clinical efficacy of existing molecular EGFR-targeted therapies against gynecologic malignancies has not been demonstrated. In this study, we investigated the antitumor effects of ligand-based EGFR chimeric antigen receptor (CAR)-T cells on gynecologic malignancies. First, we evaluated EGFR expression in patient samples using immunohistochemistry. EGFR positivity was observed in 41%, 82%, and 79% of ovarian, endometrial, and cervical cancer in patient samples, respectively. Second, we generated ligand-based EGFR CAR-T cells via piggyBac-mediated gene transfer. EGFR CAR-T cells were successfully generated with high CAR positivity and a high proportion of naïve/stem cell me

SUBMITTER: Shinagawa M 

PROVIDER: S-EPMC12317388 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature