Piezo1 induces mitochondrial autophagy dysfunction leading to cartilage injury in knee osteoarthritis.

ABSTRACT:

Background

External mechanical stress plays a pivotal role in the pathogenesis of knee osteoarthritis. Piezo1 can sense mechanical stress changes on the surface of various cell types and convert them into bioelectrical signals to regulate cellular functions. Therefore, our study aimed to investigate the role of Piezo1 in mechanically induced KOA and elucidate its underlying mechanisms.

Methods

In this study, we employed various techniques to assess the effects of mechanical stress on knee joint cartilage in vivo and in vitro experiments. In vivo, we performed Micro-CT scanning, H&E staining, and ELISA analysis on the knee joints to evaluate the degree of cartilage damage and the expression of pro-inflammatory factors. In vitro, we utilized a cell stretcher to apply mechanica

SUBMITTER: Yu L 

PROVIDER: S-EPMC12317449 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature