Human umbilical cord mesenchymal stem cell-derived exosomes enhance follicular regeneration in androgenetic alopecia via activation of Wnt/β-catenin pathway.

ABSTRACT:

Background

Exosomes isolated from human umbilical cord mesenchymal stem cells (hUCMSCs) have demonstrated the capacity to alleviate dihydrotestosterone (DHT)-induced disruptions in the hair follicle growth cycle. However, the precise role and underlying mechanisms by which hUCMSC-derived exosomes influence hair shaft regeneration in androgenetic alopecia (AGA) remains unclear.

Methods

hUCMSCs were isolated using fluorescence-activated cell sorting following enzymatic digestion with TrypLE™ Express. Exosomes derived from these hUCMSCs were purified through ultracentrifugation and subsequently characterized by transmission electron microscopy and Western blotting to confirm their morphology and protein markers. To model human AGA, mice received daily subcutaneous injections of

SUBMITTER: Yu A 

PROVIDER: S-EPMC12317459 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature