Comparative analysis of commercial and "In-House" molecular tests for the detection of intestinal protozoa in stool samples.

ABSTRACT:

Background

Pathogenic intestinal protozoa exhibit a global distribution and are significant causes of diarrhea, estimated to affect approximately 3.5 billion individuals annually. These intestinal infections continue to pose formidable diagnostic challenges. Microscopy remains the reference diagnostic method for intestinal protozoa, but is limited in terms of sensitivity, specificity and the ability to differentiate closely related species. Additionally, microscopy requires an experienced microbiologist. Emerging diagnostic methods, such as immunochromatography and enzyme-linked immunosorbent assay (ELISA), are regarded as suitable techniques for rapid screening. Molecular diagnostic technologies, particularly real-time PCR (RT-PCR), are gaining traction in non-endemic areas charac

SUBMITTER: Di Pietra G 

PROVIDER: S-EPMC12317615 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature