Can we still use the Michaelis-Menten model for enzymatic microneedle sensors?

ABSTRACT: Since the 1960s, enzymatic sensors have been vital in healthcare and environmental monitoring due to their high selectivity. Traditionally, their performance is interpreted using the Michaelis-Menten (MM) equation, which assumes idealized, homogeneous, well-mixed laboratory conditions. However, integrating these sensors with microneedle (MN) patches for wearable applications introduces challenges such as spatial and temporal variations and limited reactant availability. Applying the MM model in such scenarios can lead to dramatic errors in enzyme kinetics and biomarker estimates, risking inaccurate substrate measurements and potentially life-threatening decisions. Here, we generalize the reaction-diffusion framework for enzymatic sensors and integrate it with analytical models for MN senso

SUBMITTER: Fratus M 

PROVIDER: S-EPMC12318150 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature