Metabolic engineering <i>Saccharomyces cerevisiae</i> for <i>de novo</i> biosynthesis of hydroxycinnamoyl glycerols.

ABSTRACT: Hydroxycinnamoyl glycerols (HCGs) are a class of bioconjugates characterized by the esterification of hydroxycinnamic acids with glycerol. Owing to their diverse biological activities, HCGs are considered high-value materials with potential applications in the food, pharmaceutical, and cosmetics industries. In this study, a de novo biosynthetic pathway of HCGs was constructed by integrating a p-coumaric acid-producing module and a 4-coumarate:coenzyme A ligase - hydroxycinnamoyltransferase (4CL-HCT) module into the genome of Saccharomyces cerevisiae. Through optimization of the primary metabolic pathway, fine-tuning of the shikimate pathway, and strategic selection of integration sites, the upstream metabolic flux in the S. cerevisiae strain was engineering to e

SUBMITTER: Zou H 

PROVIDER: S-EPMC12318264 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature